While its high sensitivity and specificity facilitate simultaneous detection of a large spectrum of viruses, including those difficult to be identified by traditional methods [ 32], its capacity to detect low amounts of viral nucleic acids in some cases during viral incubation period, asymptomatic infection, or post-infectious shedding makes it difficult to interpret the results [ 30, 32].
The proposed design incorporates two metalized channels to facilitate the simultaneous detection of one group of silicone oils, which can consist of two different species.
In the present study, multiplex PCR and SYBR Green real-time PCR were developed to facilitate the simultaneous detection of three important rice pathogens, Xanthomonas oryzae pv.
Our multiplexed SRM assay has facilitated the simultaneous detection of surrogate peptides originating from 26 proteins present in cerebrospinal fluid (CSF).
Importantly, the size-dependent properties of nanoparticles (for example, gold, quantum dots) also facilitate the simultaneous detection of multiple analytes in the same system [ 12].
In this scenario, multiplex PCR has a significant advantage, as it permits simultaneous detection of several viruses in a single reaction mixture, facilitating cost-effective diagnosis [ 11].
Simultaneous detection of the status of the EGFR, HER2, and TOP2A genes may facilitate the development of effective therapeutic strategies to target gastric cancer, especially for determining those patients who may benefit most from a combination of therapies to target EGFR and HER2 together with a TOP2A inhibitor.
Simultaneous detection of several object boundaries is one of the great advantages of this method.
