These genes are the most suitable candidates for molecular cloning and development of new functional gene-target markers to facilitate marker assisted breeding.
Unlike most of the other MX cassettes, the LYS5MX cassettes are also negatively selectable (alpha-aminoadipate-resistant), which will allow the use of the LYS5MX cassettes in plasmid shuffling and will also greatly facilitate marker recycling.
The SSR markers developed would facilitate marker assisted breeding schemes for efficient introduction of desired traits.
Capture of low-abundance proteins is critical for facilitating marker discovery.
Availability of high quality genome sequence [ 5] further eases up the mining of DNA markers to facilitate marker assisted breeding programme in rice.
The use of genomic sequence from related and/or progenitor species, such as Ae. tauschii, can facilitate marker development in hexaploid wheat [ 30].
The identified locus will also facilitate marker assisted breeding of M. truncatula for increased resistance to CPA and potentially other closely related Medicago species such as alfalfa.
The identified locus will facilitate marker assisted breeding of M. truncatula for increased resistance to CPA and potentially other closely related Medicago species such as alfalfa.
The micro-collinear regions conserved between model organisms like Arabidopsis and lesser-studied organisms can also facilitate marker saturation and candidate gene searches.
One of the primers of the pair was located in an intron sequence while the other was designed within the exon to facilitate marker specificity.
