Lower RBC deformability and higher shear rates above 0.5 s−1 are found to facilitate disaggregation.
AT samples (approximately 150 μg) and cells were homogenized in 0.6 mL of QIAzol® Lysis Reagent (QIAGEN), a monophasic solution of phenol and guanidine thiocyanate which facilitates sample disaggregation and inhibits RNAses.
Equity-oriented health information systems have data collection practices that facilitate data disaggregation by relevant dimensions of inequality and across a wide selection of health topics; have the knowledge, expertise, and resources to conduct and interpret standardized analyses of health inequalities; and produce regular and high-quality reports of the state of inequality.
This is in good agreement with the proposed mechanism of enzymatic hydrolysis of cellulose by cellulases, which suggests that, rather than cellulose fibrils being slowly eroded by surface 'shaving' or 'planing', the cellulase enzymes enter through pores large enough to accommodate them, facilitating the disaggregation and fragmentation of the cellulose.
The classical cellular response to this type of stress is the induction of "heat shock" or chaperone proteins [ 42- 44] which facilitate the disaggregation of proteins and their refolding to native conformation, and/or the production of small heat shock proteins, which prevent initial protein aggregation [ 26].
Moreover, the small heat shock proteins (sHSP) of E. coli, IbpA and IbpB (IbpAB), intercalate into protein aggregates and thereby facilitate the KJE/ClpB dependent disaggregation and refolding [ 10- 12].
Lactate creates a microenvironment of low pH around the embryo to assist the disaggregation of uterine tissues to facilitate trophoblast invasion.
Regardless of the mechanisms involved, the ability of the Hsp70s to support growth and prion propagation shows that the Y. lipolytica Ssa proteins functionally interact with other S. cerevisiae chaperones and co-chaperones to protect against protein aggregation, help proteins fold, facilitate protein transport, assist protein degradation and cooperate in protein disaggregation.
Recent findings indicate that the activity of ClpB in disaggregation and refolding of heat denatured proteins is facilitated by the presence of the bacterial sHSPs, IbpA and IbpB (IbpAB) [ 11].
