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For the pENTR-V5-Ubc9 plasmid, the SUMO E2 ligase Ubc9 was inserted at the C-terminus of the pENTR-V5 vector so that cDNAs could be inserted between the V5 epitope and Ubc9, generating a fusion protein that facilitates detection of the sumoylated target protein, thereby bypassing the requirement for an E3 SUMO ligase [31].
This characteristic greatly facilitates detection of the LAMP reaction [ 9].
The objective of this study was to develop a search strategy that facilitates detection of the off-label drug use documents in EMBASE via OvidSP.
The brightest frame in the red channel is selected for the procedure to ensure maximum brightness of the abdomen, which allows a sharper contrast between wing and body and facilitates detection of the wings and wing veins in regions where body and wings overlap.
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Thus, it is possible that adaptation did not facilitate detection of the Gaussian target because the target and background were both affected in similar ways by the adaptation.
Results also show that information in independent color coding mechanisms tuned to orthogonal directions in the isoluminant plane can be combined to facilitate detection of the target.
Additionally, morphine-tolerant C57BL/6J mice, but not 129P3/J mice, exhibited a large increase in the frequency of tail flicks during the first second following the baseline nociceptive response which may facilitate detection of the response during the tolerant state.
This is in contrast to electron ionization (EI) where ionization typically results in extensive fragmentation, and diagnostic ions and/or spectra need to be known a priori to facilitate detection of the analytes in the raw data.
A Myc-tag was constructed at the N-terminus of Smad7 transcript to facilitate detection of the transgene expression in the animals (Figure 1A).
To facilitate detection of the two proteins, SΔC was tagged with a Myc tag and eIF3f with a Flag tag at the N-termini, respectively.
We also generated a variety of Entry vectors that encode epitope tags at their N-terminus (GFP, V5, FLAG) or C-terminus (GFP, V5-His) in different reading frames to facilitate detection of the proteins of interest.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com