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The minor groove binding induces bending of the DNA by 80° and facilitates base pair melting.
Sequences were resolved using ABI 3730 XL DNA Analyzer (Applied Biosystems) and analyzed by the Phred, Phrap and Consed package (23), which facilitates base calling from sequencing trace files, sequence quality assessment, and assembly.
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However, appropriate choice of the packing pore size facilitates base-line separation of particular sample constituents.
The program aligns and scores (percent sequence similarity) sequences facilitating base by base comparison of replicate samples as well as comparison of sequence samples against an established standard.
The resulting tetrahedral phosphonyl adduct adversely affects the ability of H468 to facilitate base mediated C-O bond cleavage [15], which leaves this residue in a state where it cannot activate the water molecule required for hydrolytic desphosphonylation [16].
These proteins are required for the assembly of U1 snRNP on the splice sites, primarily by facilitating base pairing between U1 snRNA and the nucleotides around the 5' exon/intron junction.
Most sRNAs act by base pairing with a target mRNA, modulating its translation and/or stability; many of these RNAs share only limited complementarity to their mRNA target, and require the chaperone Hfq to facilitate base pairing.
In many instances of trans-encoded sRNA regulation, the sRNA chaperone Hfq is required to facilitate base pairing between discontinuous stretches of limited complementarity between the sRNA and its target (Brennan and Link 2007).
The post-transcriptional regulator and RNA chaperone Hfq has been shown to facilitate base pairing between bacterial sRNAs and mRNA targets [ 3]. sRNAs modulate the expression levels of target genes in response to specific environmental changes.
While upon exposure to Hydralazine, neither expression of deaminases Aicda, Apobec1, or Apobec3 was significantly regulated (Supplementary Fig. 7C), Tdg and Smug1 expression (which facilitate base excision repair) were significantly increased upon Hydralazine treatment (Fig. 6A and Supplementary Fig. 7D).
This was intended to be facilitated based on the audit of evidence-based practice learning gathered as part of the baseline data collection.
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