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The addition of channels facilitated cell migration and subsequent deposition of cartilage-specific matrix into more central regions of these explants.
We found altered expression of 24 genes (7.5%) that facilitated cell migration at the Inc phase, but only 3 at Pk and Rec phases combined.
Decreased gene expression of integrin receptors, ligands, and other matrix components could result in a poor connection between cells and the substratum, that in turn could have contributed to a transformation phenotype and further facilitated cell migration and invasion.
Moreover, the upregulation of poFUT1 facilitated cell migration and invasion compared with the mock control, as analyzed by transwell cell migration assay and matrigel cell invasion models.
Bone marrow cells from rats and human embryonic brain cells show a facilitated cell migration [ 153, 154], while bone marrow CD34+ cells have a lower migration potential in simulated microgravity [ 155].
Additionally, the transwell assay was conducted to evaluate the cell migration activity, with the results that forced expression of miR-612 inhibited CRC cell migration, whereas suppression of miR-612 facilitated cell migration.
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The ability for the scaffold to provide (i) an ideal environment for the cells to migrate, (ii) porosity that facilitates cell migration and crosstalk, and (iii) a biocompatible material are necessary to achieve proper healing in vivo.
Actin regulators facilitate cell migration by controlling cell protrusion architecture and dynamics.
For instance, MMP-2 can cleave the adhesive contacts and cellular networks that cells use to adhere to their basement membrane, facilitating cell migration [14], [34], [35].
We report here that Sema3E from tumor cells can act on themselves through Plexin-D1 to induce EMT and concomitantly facilitate cell migration and malignant progression.
A matrix composed of shorter fibers may facilitate cell migration by effectively reducing the apparent viscosity of the tissue as cells may be less globally interconnected.
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