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This is consistent with the recent observation that overexpressions of miRNAs induce pluripotency [ 10] and miRNAs also facilitate stem cells differentiation [ 16].
ECM proteins, such as collagens and laminin, mixed with growth factors were potent to facilitate stem cells differentiating to hepatic lineage [ 13].
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To determine if transient expression of self-renewal genes directed by mRNA-carrying NPs can facilitate stem cell expansion, we loaded α-CD105-targeted NPs with synthetic mRNA encoding MSI2 and added them to cultures of HSCs.
There is a great possibility that the superior adhesion and proliferation of stem cells, including nerve stem cells, on a nanofibrous scaffold will be able to facilitate stem cell based therapy clinically.
Stem cell niche engineering is an emerging modality that seeks to facilitate stem cell recruitment, aid the effectiveness of exogenous stem cells, and ultimately result in the reestablishment of stable stem cell populations in support of wound healing and normal skin homeostasis.
Therefore, it is important for biomaterials to be able to orchestrate the biochemical and biophysical cues to facilitate cell cell and cell ECM interactions to facilitate stem cell therapy.
Polycomb complexes facilitate stem cell self-renewal and pluripotency, and both Bmi1, a component of the PRC1 polycomb complex, and Suz12, a component of the PRC2 polycomb complex, have been identified as targets of miR-200 family members [ 51- 53].
Dissecting the molecular basis of epicardium and EPDC specification would not only help to understand developmental processes and mechanisms of cardiovascular diseases but would also facilitate stem cell-based regenerative therapies to regenerate and repair injured cardiac tissues.
Recombinant human PLCζ protein could potentially also be used in regenerative medicine approaches via generation of parthenogenetic embryos and blastocysts that may facilitate stem cell derivation and differentiation.
Because these approaches require the delicate composition of the contrast agents and the "cell-uptake enhancers," novel MR contrast agents that facilitate stem-cell labeling have developed rapidly in recent years [6, 7].
It is noteworthy that these films can be deposited on a wide range of substrates with different shapes, sizes and composition, and can load other growth factors for facilitating stem cell differentiation toward specific lineages.
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