The N-terminal Gly facilitated labeling.
The PCR products were further amplified using aminoallyl-dUTP incorporation to facilitate labeling with the fluorophores Cy3 (parental A2780) or Cy5 (A2780 following various "rounds" of cisplatin treatment).
By facilitating label-free quantification, second-generation proteomics techniques enable flexible proteomic workflows.
As a key feature, its structural design facilitates both, labelling with fluorine-18 at its terminally fluorinated butynyl moiety and carbon-11 at its methyl ester function.
Recently, we have seen the emergence of RTCA (real-time cell analysis) platforms which facilitate both label free and operator independent investigation of cell behaviour by monitoring the cells in physiologically relevant conditions [ 26, 27].
However, in order to further facilitate the labelling procedure for 177Lu and 90Y, improve ligand pharmacokinetics, and potentially obtain radiometalated derivatives with higher affinity, we substituted DOTA in DOTA-FFK(Sub-KuE) by 1,4,7,10-tetraazacyclodocecane,1- glutaric acid)-4,1- glutarictic acid -4,7,10-triacetic acid -4,7,10-triacetic acid -4,7,10-triacetic
We also used rabbit polyclonal antibodies raised against Mcm-5 (Williams et al, 1998) to facilitate double labelling, and a rabbit polyclonal against phosphohistone H3 (Upstate Biotechnology, Lake Placid, NY, USA).
The what and the how: Information-seeking pointing gestures facilitate learning labels and functions.
A mix of dT20 and random nonamer was used to prime the reaction in order to maximize the reverse transcription of both polyadenylated and non-polyadenylated RNAs, and to facilitate the labeling of the entire RNA sequence.
In this step, the amplification protocol was modified as described in Schuelke (2000) to facilitate fluorescent labeling of the PCR products by 6-FAM (Applied Biosystems, Foster City, California, USA).
