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One of obstacles to this end and to the large scale production of the enzyme has been its facile expression in a bacterial host.
Genetically engineered variants of human lysozyme represent promising leads in the battle against drug-resistant bacterial pathogens, but early stage development and testing of novel lysozyme variants is constrained by the lack of a robust, scalable and facile expression system.
We created yeast-based cellular systems that permit facile expression of proteases and protease-activating proteins in combinations that reconstitute entire mammalian pathways in these simple eukaryotes.
A number of microbial P450s have already been characterized and offer advantages in terms of their high catalytic rates and facile expression in microorganisms.
In addition, PNGase H+ exhibited better release efficiency over N-glycans without core α1,3-fucose compared with PNGase A. The facile expression, non-glycosylated nature, unusual pH optimum and broad substrate specificity of this novel type of N-glycanase makes recombinant PNGase H+ a versatile tool in N-glycan analysis.
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Caro, L. N. et al. Rapid and facile recombinant expression of bovine rhodopsin in HEK293S GnTI− cells using a PiggyBac inducible system.
The 2A oligopeptide is emerging as a highly effective new tool for the facile co-expression of multiple proteins in a single transformation step, whereby a gene encoding multiple proteins, linked by 2A sequences, is transcribed from a single promoter.
Their sayings are facile and expressions are upside-down.
Walsh, D. M. et al. A facile method for expression and purification of the Alzheimer's disease-associated amyloid beta-peptide.
Cell-free synthesis not only offers excellent throughput in the generation of enzymes, it allows facile integration of expression and analysis of enzymes, greatly accelerating the process of enzyme discovery.
One retained too much elegance; another was marked, on looking again, by a psychology too facile, read in the expression of grief, pity or love.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com