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Finally, the scaling model is constructed by the N f pairs {x i,MAX,x i,MIN} and the pair {y i,MAX,y i,MIN}.
In addition, the F atoms of the four dNTP/dF pairs have four ordered water molecules located within 3.2 Å of its radius.
Compared to the structures of dNTP/dT-containing ternary complexes, the shapes of the nascent dNTP/dF pairs differ from those of the dNTP/dT pairs.
We then determined and compared the structures of eight RB69pol ternary complexes, four with each of the dNTP/dF pairs and another four with each of the dNTP/dT pairs.
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In particular, the dGTP/dF pair has a direct interbase HB, while the dGTP/dT pair adopts wobble geometry.
As for the dTTP/dF pair, the base of the incoming dTTP did not stack nearly as well with the penultimate base pair compared to the adenine base of the dATP/dF pair.
In the 8-task environment, the results are in line with the experiments performed using the (R, F) pair.
Any pair that included the operator with less than 1 year experience was considered inexperienced, and the microscopists E and F pair had the highest expert rank.
As shown in panels C and D of Figure 3, the geometry of the dATP/dF pair is very similar to that of the dATP/dT pair.
In the case of the dGTP/dF pair, the distance between C of dF and N of dGTP is 3.32 Å.
Results correspond to the statistic of the tests (Kruskal-Wallis (X2), Wilcoxon (W), and analysis of variances (F)) paired with the corresponding probability (p).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com