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The third design overcomes the problem by ending the fibres at the end of the eye section.
Far from placing Wilder in his pantheon of the greatest directors, Sarris quarantined him within his starkly named Less Than Meets The Eye section, alongside other figures of contestable quality such as John Huston, Lewis Milestone and Fred Zinnemann.
Hematoxylin eosin (H&E) staining of the entire eye section showed no obvious abnormality for mice over expressing Wt, D606G and Del601 640.
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Finally, experimental results obtained for the cell application to qualitative elemental imaging of structurally complex tissue samples (e.g. eye sections from a native frozen porcine eye and fresh flower leaves) demonstrate that working in cryogenic conditions is critical in such type of direct sample analysis.
To up the drama for nighttime, add a few lashes on the outer eye sections and bottom.
Immunohistochemistry was performed at room temperature in the same way as it was done with the mice eye sections.
Subsequently, at the development stages of 3 and 5 dpf, eye sections of WT fish and morphants were further examined (Fig. 3E).
To minimize the error in measurement resulting from samples at different location in the eye, sections were chosen from similar depths within the retina as judged by the size of the section.
The antibodies listed above in the immunocytochemistry section were also used on eye sections together with a cocktail of antibodies generated against human-specific markers (HSM) to identify human cells (the Oka blood group antigen, mouse TRA-1-85, 1∶10 (a kind gift from Peter Andrews, University of Sheffield, Sheffield, UK) together with mouse human nuclear antigen, 1∶1000, Millipore).
Next, we immunostained human eye sections using the same antibody.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com