Exact(4)
ovo is an eye progenitor marker.
By contrast, fst RNAi caused the opposite phenotype of reduced ovo+ eye progenitor formation.
Thus, eye progenitor cells, which are labeled with Smed-ovo [ 54], are not present in Smed-meis-like RNAi Smed-meis-like RNAi Smed-meis-like RNAi
Although act-1 RNAi) act-1 RNAisplanimalsrmal ovo+ eye progenitor numbers prior to amputation, increasedisplayed as compared to conormal were present fovo+wing amputation.
Similar(56)
Additionally, not all progenitors express piwi-1 mRNA; for example, in the trail of eye progenitors, piwi-1 mRNA expression is limited to the posterior-most cells.
(C ) act-1 RNAi) act-1 RNAisplanimalsgreater indisplayedf ovo + eye progenitors compared to controls 2 days after head amputation (n = 24, p<0.0001, two-tailed t -test).
Head amputation normally induces neoblasts to produce ovo+ eye progenitors (Lapan and Reddien, 2012), but this process failed in fst RNAi) animals.
(D ) fst RNAi) animals lacked eye progenitors following head amputation as assayed with RNA probes for ovo +/ smedwi-1 + cells (p<0.001, two-tailed t -test).
We use the same progenitor term used in Lapan and Reddien (Plos Genetics 2011) and Lapan and Reddien (Cell Reports 2012) describing the sp6-9 + and ovo + cells as eye progenitors, respectively.
During regeneration, eye progenitors express conserved TFs ovo, six-1/2 and eya at a distance from the eye primordium, forming a trail of cells and undergoing changes in gene expression as they migrate toward the primordium, where differentiation markers begin to be expressed (Lapan and Reddien, 2011; Lapan and Reddien, 2012).
For some tissues like the eyes, progenitors are easily demarcated as a trail of sp6-9+ and ovo+ posterior to the eyes.
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