Exact(12)
We measured loss of intravascular fluorescence activity using an eye assay that we established previously [18].
In the eye assay Tlr4 was a strong suppressor, while Tlr3 and TlKG03609 were both weak suppressors.
Both of the Tl alleles are hypomorphs and neither was a strong suppressor in the eye assay.
Since both cact and myd88 are thought to be canonical cell autonomous components of the Tl→NFκB signaling pathway a plausible explanation is that these genes are not haploinsufficient in the eye assay.
The Tl allele that is the strongest suppressor in the eye assay, Tlr4, appears to extend the life span of UAS-Aβ42/elav-GAL4 flies by about a quarter.
We set out to determine if the limits in cellular plasticity that we observe in our ectopic eye assay is a reflection of either not expressing individual RD genes at a high enough level or by not expressing the correct combination of RD network branches.
Similar(48)
It is important to note these phenomena since changes observed in the eye assays could be attributed to a general effect on the vascular system.
The advantage of the Fabp-eye-assay is that cardinal vein injection is not performed.
The advantages of the FITC-eye-assay are that it can be performed on any strain of fish where the FABP-eye-assay is dependent on the Fabp transgenic fish and that the genetically mutant fish can be screened [ 48].
The FITC and the Fabp-eye-assay systems both have advantages and disadvantages when compared with each other.
Tg l-fabp:DBP:EGFP) fish, which were iniTg l-fabpnerateDBP EGFPualize the blood brain barrier in vivo are used in the fish-eye-assay [ 47].
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