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Ethanol (5%) was added to the extrusion medium immediately before cell extrusion.
Then, each worm was placed for 3 min in a 15-ml polypropylene tube containing 30 ml of cold extrusion medium [Nacl (71.2 mM), EDTA disodium salt (6.7 mM), GGE (50.4 mM), ethanol (2% v/v) and a supplement of antibiotic and antimycotic agents: penicillin G sodium salt (100 U/ml), streptomycin sulphate (100 μg/ml), amphotericin B (25 mg/ml)].
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The process of P solubilisation was caused most likely by proton extrusion and medium pH lowering.
The subsequent [Ca2+]i decrease to resting levels is operated by active extrusion to extracellular medium or reuptake into intracellular organelles.
By using the biofabrication method presented in our recent framework study18, we generate tissue strands that are bioprintable, facilitate rapid fusion and maturation through self-assembly, enable bioprinting in solid form, do not need a liquid delivery medium during extrusion, and do not require a support molding structure during bioprinting for cell aggregation and fusion.
The Drickamer anvils are normally used with a containment ring to limit extrusion of the pressure medium under load.
Subsequently, the 10 ml crude liposome dispersion was sized by multiple extrusion at 60 °C using a medium pressure extruder (Avestin, Mannheim, Germany) equipped with two stacked polycarbonate membrane filters, one with a pore size of 200 nm on top of one with 100 nm pores.
This might be explained by the need of stressed plants to pump more Na+ out of the cell, either via loading of Na+ into the xylem or via extrusion of Na+ back to the growth medium.
After 14 hours of culture in milrinone-free medium, exogenous Sgo1 overexpression significantly decreased extrusion of the first polar body (PBE), from 82.2±3.3% in the control to 55±2.4% in the injection group (P<0.05) (Fig. 3B).
The effects of different process parameters, such as extrusion pressure, number of cycles and viscosity of the medium were studied on a change in average surface roughness (ΔRa) and material removal.
For planktonic (free-floating) prokaryotes, proton extrusion does not alter the pH of the outside medium.
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