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To address this problem, we removed an extraneous target by pretreating genomic DNA with a restriction enzyme.
Digestion of the template eliminated amplification of the extraneous target and allowed the identification of four additional mutant alleles compared to untreated template.
To remove a target from TILLING assays, sequence information was used to choose a restriction enzyme that cut once within the extraneous target (sequence data from primer testing was sufficient to identify an appropriate enzyme; cloning was not necessary).
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We wondered whether an alternative to extensive primer testing would be to eliminate amplification of extraneous targets from the genomic DNA.
"There is a huge, extraneous object right off our harbor.
And worst of all, extraneous lawsuits targeting companies attempting to share helpful information with workers regarding taxes, insurance, and retirement accounts, are punishing the very individuals these groups falsely espouse.
By contrast, the Trump boycotts, from both the left and the right, have been driven by issues extraneous to the targets' core business practices.
These extraneous genes provided expansion targets for the resident IS element population.
In the first row (top), there are no extraneous stimuli near the saccade target.
Auto- and cross-correlation of spectral data facilitates the measurement of diffusion constants for particles ranging in radius from 50 to 500 nm while discriminating signal associated with the target analyte from extraneous fluctuations.
In this study, we have grouped together all extraneous acoustic parts in a single target class called residual.
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CEO of Professional Science Editing for Scientists @ prosciediting.com