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Since Wnt2 is a secreted protein, genetic rescue could have occurred by expression from extraneous cell types.
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Since single cells are targeted to the electrodes without nearby extraneous cells, individual cell responses can be unambiguously recorded.
Furthermore, to demonstrate the enhanced sensitivity of mutant strains to extraneous oxidative stress, cells were subjected to hydrogen peroxide treatment.
Furthermore, the data can be heavily skewed by various extraneous factors such as cell passage number, growth conditions and state of confluency among others.
However the mRNA translation process is closely regulated at multiple levels and is also subject to noise, and further, synthetic circuits may be subject to extraneous interactions in the host cell(s).
Dewaxed sections were stained for deposited sulfated proteoglycans (sGAG) using alcian blue (pH 2.5), collagen using picrosirius red, and with haematoxylin and eosin (H&E) for cell nuclei and extraneous matrix, respectively.
The integration of DNAfs and Cfs is stable and presence of extraneous DNA was demonstrable in single-cell clones developed from treated cells which had undergone numerous cell divisions.
After washing off extraneous dyes with HBSS, the cells were treated with increasing doses of ZnCl2 and the fluorescence from cells was immediately measured using a Victor 3 V1420 multilabel plate counter (Perkin Elmer) with measurements every 5 min for 30 min. ATP production rate was determined following the previously described protocol (47).
As expected, when extraneous CaMCA1 was introduced into these cells, the caspase activity and cell sensitivity to H2O2 were resumed.
For analysis of sensitivity to extraneous oxidative stress, the yeast cells were spotted on selective media containing 1 m m H2O2.
The criticality of ROS modulatory proteins comes into the fore when cell is exposed to extraneous stresses.
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