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Extracts were concentrated to 100 μl using a vacuum concentrator (SpeedVac®, Thermo Fisher Scientific, MA, USA).
The extracts were concentrated such that almost all the extracting solvent was removed then transferred to pre-weighed beakers and allowed to dry completely in the fume cupboard.
The extracts were concentrated (1 100) in double-distilled water to allow extraction of active compound onto the C18 cartridge.
All extracts were concentrated under vacuum until dryness and re-dissolved to a standard concentration of 5 mg ml-1 in MeOH.
The extracts were concentrated and strained through a 0.45 μm filter, and the entire extraction process repeated twice.
Extracts were concentrated to 1.0 mL and further cleaned using a solid-phase extraction cartridge (Supelclean ENVI-Florisil, 6 mL, 500 mg bed weight; Supelco).
The methanol extracts were concentrated with rotary evaporator.
The extracts were concentrated under reduced pressure to a constant weight.
The extracts were concentrated in a rotary evaporator and lyophilized (Al-Hulu et al. 2012).
MAE extracts were concentrated and derivatised prior to separation and quantification by gas chromatography with electron capture detection.
The combined extracts were concentrated to approximately 2 mL under a stream of argon over 36 h.
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