Using the Xenopus egg extract system, we have investigated regulation of the Rad1/Hus1/Rad9 complex.
Using the Xenopus egg extract system, we investigated the involvement of DNA replication in activation of the DNA damage checkpoint.
The amount varies depending on the amount of prepressing, the type of material being extracted, and the efficiency of the extracting system.
These include a protocol for the preparation of nucleoplasmic extract (NPE), which is a soluble extract system useful for studying nuclear events such as DNA replication and checkpoints.
A nonlinear least-squares technique is then implemented to extract system parameters from laser vibrometry data of the diaphragm motion.
The extract system is ideal because it provides the complexity of total cytoplasm, yet is open, allowing control of cell cycle progression, specific inactivation of individual components, high-resolution observation, and biochemical and biophysical approaches.
Using the Xenopus egg extract system, we show that lesions induced by UV irradiation and cis-platinum cause the functional uncoupling of MCM helicase and DNA polymerase activities, an event previously shown for aphidicolin.
Previous work on the responses of mitogen-activated protein (MAP) kinase cascade components in a Xenopus oocyte extract system demonstrated that p42 MAP kinase (MAPK) exhibits a sharp, sigmoidal stimulus/response curve, rather than a more typical hyperbolic curve.
Oscillating water column based wave energy extracting system has a low efficiency due to the poor performance of its principal power extracting component, the bidirectional turbine.
In vitro systems such as the Xenopus egg extract system, have played, and still play, an important role in deciphering the molecular details of these processes.
