αV, β1, β3 or β5 integrin subunits and indicated combinations were knocked-down by siRNA in U-87 MG and SKOV-3 and protein extracts were submitted to immunoblotting using specific antibodies.
For this, whole adrenal protein extracts were submitted to western blot analysis (Fig. 1A).
Cell extracts were submitted to immunoblot analysis of phospho-ERK as a measure of receptor activation.
Thus 12 stool bacterial DNA extracts were submitted to pyrosequencing analysis, with two replicates of each.
Written informed consent was required for the samples collected in Catalonia and were also anonymized; then, DNA extracts were submitted to the laboratory in Santiago de Compostela were the genotyping was carried out.
Tissue extracts were submitted to proteomic preparations for 2D-IPG.
Diluted DNA extracts were submitted to the University of Minnesota's BioMedical Genomics Center in a 96-well format for sample quality assessment and SNP genotyping using the Sequenom MassARRAY® technology.
To check the availability and quality of the DNA, extracts were submitted to multiplex PCR using the specimens control size DNA ladder (In Vivo Scribe Technologies), which enables amplifying 100, 200, 300, 400, and 600 bp DNA fragments [ 29].
The three extracts were submitted to phytochemical reactions for detection of the fixed acids, alkaloids, anthocyanins, anthocyanidins, aurones, antranols, quartenary bases, catechins, chalcones, cyanogenic heterosides, coumarins, flavones, flavonols, flavanones, flavononols, phenols, quinones, resins, saponins, steroids, triterpenoids and xanthones.
