Sentence examples for extracts stained from inspiring English sources

Parasites were grown in vitro in the presence of extracts, stained with RNA sensitive dye, and imaged at timed intervals with the BD Pathway HT automated confocal microscope.

293T cells were transfected as indicated in Fig. 4A and the protein extracts were stained with anti IL-1RI abs.

The nuclear extracts were stained by the anti-lamin C antibody as a nuclear lamin marker but not by the anti-α-tubulin antibody as a cytoplasmic marker.

Leukocytes from spleen extracts were stained with APC-labeled anti-CD3 antibodies (clone 145-2C11) for 30 min and then washed with 1 × PBS buffer for THG microscopy (137 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, 2 mM KH2PO4, pH = 7.4).

Brains were then extracted and stained with tetrazolium chloride to calculate the infarct volume.

Another SDS-PAGE was run with 10 μg of protein extract and stained with Coomassie Blue [ 46].

Standard proteins and the cell extract were stained with 0.1% Coomassie brilliant blue R-250, 50% methanol, and 10% glacial acetic acid.

Organs were then extracted and stained by H&E (i); scale bars represent 10 μm; arrows indicate infected red blood cells or (ii) and (iii) analysed by electron microscopy.

First, it was shown that extracts from stable mt-PCPH-transformed NIH3T3 cells presented GDPase activity, using in-gel activity (performed by native gel electrophoresis of the extracts and staining for inorganic phosphate) and standard GDPase assays [ 8].

The absorbance of the extracted stain was then measured at 562 nm and compared to an alizarin red standard curve.

After 15 min at room temperature and shaking, 10 μl from the extracted stain were transferred to a 96-well plate and diluted 10 folds with CPC solution.