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Most of these were identified by immunoblotting soluble allergen extracts separated by electrophoresis with patient sera or monoclonal antibodies [18].
Hexokinase levels were determined from total, unfractionated neuronal extracts separated on 12% SDS-PAGE gels and blotted onto nitrocellulose membranes.
Heme stain profiles of whole cell extracts separated by SDS-PAGE showed that expression of CymA and other Mtr proteins was not affected by the menC insertion (data not shown).
In KAx-3 extracts separated by SDS-PAGE, a single band of AdcA of approximately the predicted size (65 kDa), and that is absent in the adcA knockout strain, was detected by both antibodies.
To evaluate the selectivity of depletion, we first analyzed the pattern of immunoblotted proteins from undifferentiated and differentiated primary human keratinocyte extracts separated under non-reducing conditions (Figure 5A).
Several sub-fractions (spots) of extracts separated on TLC plates also exhibited powerful radical scavenging activity.
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Protein separation was achieved by SDS-PAGE using either 15% (histone extracts) or 10% (whole cell extracts) separating gels.
Immunomodulatory effect of water soluble extract separated from mycelium of Phellinus linteus on experimental atopic dermatitis.
The [14C]-sugars formed were extracted, separated and then purified by paper chromatography.
With this LPME-chip-HPLC system, series of samples were automatically injected, extracted, separated, and detected without any operator interaction.
Dorsal right hippocampi were removed, membrane proteins were extracted, separated by blue-native gel electrophoresis with subsequent immunoblotting using brain receptor antibodies.
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