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Highly efficient molecularly imprinted polymers (MIP-CDHB) may be used as sorbents in solid-phase extraction for purification extracts of tissue and preconcentration ZON and α-ZOL.
NF-κB activity was evaluated by binding of 60 µg of tissue extracts of tissue or 8 10 µg of nuclear extracts from cells, as described [18].
Nuclear extracts of tissue samples and cells were used as inputs for DNA-protein binding purification.
Cytosolic protein extracts of tissue samples from FTC, FTA, PTC, and reference thyroid were separated by 2-DE.
Protein extracts of tissue samples were prepared as per manufacturer's instructions (EMD Millipore, Billerica, MA; catalog No. 48-611).
The levels of NFA and HFA sulfatide molecular species in lipid extracts of tissue samples were determined by ESI/MS using shotgun lipidomics for sphingolipid analysis (49, 50).
Similar(51)
It had been demonstrated, with extracts of tissues and cells now known to express the human NAT1 gene, that tamoxifen is an inhibitor of human NAT1 [15 17].
The level of thiobarbituric reactive substances (TBARS) was assessed in butanol-extracts of tissue homogenates (10% w/v prepared from frozen samples), according to the manufacturer's protocol (Northwest Life Science Specialties, LCC, Vancouver).
Tissue sphingomyelin contents were estimated by the method of Hojjati and Jiang [21] from ethanol extracts of powdered tissue.
In conclusion, the organic extracts of gonadal tissue showed the most inhibitory activity on the growth of S. mutans and S. sobrinus.
In this study, we tested the applicability of cast polypropylene (CPP) membranes in AMAC and evaluated the optimized dialysis procedure for the cleanup of extracts of fish tissue.
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