Two ampoules with 1.26 mg extract of grass pollen (Gräsermischung, 450,000 standardized quality units; ALK-Scherax, HamburGermanyany) were dissolved in 1 mL of phosphate buffered saline (PBS) buffer (Sigma-Aldrich Co, Chemie GmbH, Steinheim, Germany).
We now assessed the binding of seven of our IgE-derived human antibody fragments, to pollen extracts of several grass species.
Aqueous extracts of the forage grass L. perenne contain a potent elicitor of plant defence reactions.
Lipophilic extraction was carried out by Soxhlet extraction in a reflux condenser for 6 hours with 99 w/w% ethanol on water extracted samples of grass and grass silage.
Past studies of the Fab of clone 5 indicated that it reacted strongly not only to components of timothy grass (Phleum pratense) pollen but also to pollen extracts of other grasses 25, 33.
Patients were randomized to receive once-daily sublingual tablets containing either placebo or extracts of 5-grass pollens (A. odoratum, D. glomerata, L. perenne, P. pratense and P. pratensis) at doses of 100 IR, 300 IR, 500 IR; 300 IR corresponds to 25 μg/tablet of Group 5 major allergens.
Gold nanotriangles synthesized using the leaf extract of lemon grass plant do not show any cellular toxicity to non-phagocytotic cancerous cells while the gold salt precursor showed acute toxicity to the cells.
The extract of Centipede grass (CG) (Eremochloa ophiuroides) was supplied by Korea Atomic Energy Institute (KAERI).
We used the same standardized extract of five grass pollen allergens as for the skin test (Stallergenes Laboratory) The initial allergen concentration of standardized pollen extract was 0.1 or 0.2 IR (index of reactivity), as previously described by Aubier et al. (1998).
Today such healers use a local sponge or type of grass to extract yat.
Allergen-specific immunotherapy (IT) was introduced in 1911 by Noon who was the first to report a successful application of grass pollen extract in patients with seasonal allergy.
