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Total polyphenols are relatively high in FPR and LPR extracts in comparison to BSG extract.
However, this observation may simply be an artefact of the relatively large levels of template molecules that we have in our extracts, in comparison to those found in other studies of degraded materials, and thus we recommend that future studies directly address this issue.
This highlights the equal effectiveness of aqueous and acetone extracts in comparison to essential oils.
In the present study EDE exhibited the best performance with lowest IC50 among all extracts in comparison to EDTA used as standard.
Staining with antibodies against allograft inflammatory factor 1 (also known as Iba1) did not reveal augmented activation of microglia in mice injected with human brain extracts in comparison to mice injected only with PBS (Additional file 7).
The results evidenced an increase in the activity of these extracts in comparison to the methanolic extract; but the main activity was obtained with chloroform with a MIC value of 90 μg/mL.
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Under these conditions, close to 80% of the oil was extracted in comparison to 10 h required for the Soxhlet process.
Prolonged scCO2 extraction to 9 h resulted in 620 μg of glycolipids and 875 μg of phospholipids in a mg of extract in comparison to (1.5 h) 55 μg and 15 μg of glycolipids and phospholipids, respectively.
For calculating the catalytic rate constant, the volume of the catalyst was reduced to 1/10th (0.01 mL, synthesized with 100 mg L−1 leaf extract) in comparison to the previous reaction to slow down the reaction rate.
They noted highest IC50 value for crude methanol extract in comparison to ethyl acetate fraction.
PIDG provides an indication with regards to the strength of antifungal activity of the extract in comparison to the positive control (0.12% w/v CHX).
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.
Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com