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To further demonstrate the involvement of JAK2 in contact-dependent STAT3 activation, we directly assessed JAK2 phosphorylation in cell extracts from the various treatments using SDS-PAGE and immunoblotting using anti-phosphotyrosine JAK2.
The sequential extracts from the various specimens were subjected to hyaluronan zymography in acidic conditions.
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We compiled our table using the values we extracted from the various data sets.
Genomic DNA was extracted from the various cell lines and subjected to Eco32I digestion and Southern blot analysis.
The transposable elements were extracted from the various types of masked repeats.
6 Table 1 shows the population, years, sample size, and variables extracted from the various data sources.
For this purpose, tetrapyrroles were extracted from the various E. coli strains and separated using reversed phase chromatography as described in the Experimental section.
For Southern blotting, 20 μg genomic DNA was extracted from the various trypanosome cell lines and digested with the appropriate restriction enzymes, before separation on an 0.8 % agarose gel.
Genomic DNA and RNA extracted from the various MPM cells were used to detect mutations, polymorphisms and mRNA expression levels of genes potentially affecting drug activity, as depicted in Table 1.
Soluble proteins were extracted from the various organs by grinding in 20 mM Hepes, pH 7.0, containing 1 mM EGTA, 10 mM vitamin C, and PVP PolyclarAT (100 mg/g fresh weight).
It not only allows us to deal with how much food can be sustainably extracted from the various resource systems to feed ourselves but addresses the deeper and, we believe, more important question: how many of us should there be to feed?
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