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In carbonate extracts, an increase in the extractability of pectic arabinogalactan epitopes was evident but more consistently in the biomass incubated with the parent and deletion mutant strains.
In liver extracts, an increase in glutathione in animals exposed to 25 µg BPA suggested a possible modulation of this detoxification pathway and also a hyperproduction of pyruvate, a key metabolite involved in the Krebs cycle, glycolysis, and glycogenesis.
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In the model of human bronchial epithelial cells treated with cigarette smoke extract, an increase of intracellular ROS was observed, and the activation of autophagy had a deleterious effect promoting the death of epithelial cells [ 26].
Moreover, western blot analysis of spermatogonia protein extracts showed an increase of about 2 fold for Kit, Scp3 and Stra8 with respect to extracts from cells cultured at unit gravity (Fig. 2F; Table 1).
Many of the herbal extracts showed an increase and decrease in calcium signal using the CytoFluor Multi-well plate reader.
Comparison of MMEJ in thymic cell-free extracts showed an increase in the efficiency of joining with an increase in length of microhomology (data not shown).
(D ) High pressure liquid chromatography (HPLC) analysis of cell extracts showing an increase in PI 4,5 P2 levels in patient cells (Student's t test, *p = 0.01).
The results showed that only B. coccolobifolia and B. ligustrifolia extracts induced an increase in the number of revertant colonies relative to the negative control, indicating mutagenic activity.
RKO cells treated with Hyrtios sp. extracts showed an increase of p53 protein levels and also increased expression of p21 protein which is encoded by a p53 target gene.
In the presence of lectin, the different extracts elicited an increase in splenocyte proliferation by 139.8%, 227%, 236.88% and 293.44% at 1 mg/ml, respectively, compared to the untreated cells.
In the present study, it was observed that P. indica root crude aqueous extracts caused an increase in membrane lipid oxidation, and induced phosphorylated-p53 and p21expression in GBM8401 and HeLa cells.
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