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All 68 DNA extracts amplified under these conditions.
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Ancient fish DNA could be successfully extracted, amplified and sequenced.
Herein, the 18S rDNA of the strain L45 was firstly extracted, amplified, and sequenced.
RNA was extracted, amplified and subjected to microarray analysis.
Genomic DNA was extracted, amplified and sequenced as per [48].
Two pools of viral resuspension were extracted, amplified and, cloned.
RNA was extracted, amplified and assessed for quality as described for murine samples.
The DNA was extracted, amplified, cloned and sequenced using standard methods and is detailed in File S1.
PCR products from nematode homogenates were cloned into pGEM-T Easy (Promega), transformed into E. coli XL1-Blue, extracted, amplified and purified according to standard procedures, and sequenced as described below.
olsoni 1) was independently extracted, amplified and sequenced in two dedicated ancient DNA laboratories for their authentication, and 3) about 50% of the fragments for each specimen have been replicated twice (Figure S1).
Following histological assessment of the tissues, the stromal compartment from both PIN lesions and invasive prostate tumors was selectively removed by LCM (Figure S1) and the RNA was extracted, amplified and subjected to microarray analysis.
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