Exact(48)
Plant extracts (1 g leaves in 3 ml extraction buffer [20 mM Tris-HCl, pH 8.0, 138 mM NaCl, 3 mM KCl, 1 mM PVP, 0.05 % Tween 20]) were treated with DNase.
Extracts 1 to 6 were resolved into two fractions, referred to as anionic and cationic, by anion-exchange chromatography.
Extracts (1 mL) were mixed with NaNO2 solution (4 mL, 1 5, w/v) and incubated at room temperature for 6 min.
Extracts 1 to 6 were further resolved into two fractions, named anionic and cationic, by anion-exchange chromatography (Additional file 1: Figure S2).
Furthermore, this biosensor was utilized for GSH detection in diluted HeLa cells extracts (1%) samples with satisfactory results, which can meet low concentration detection for GSH with a good linear relationship in the range from 0.05 to 1.2 mM.
In order to quantify the compounds, samples of crude extracts (1 mg/mL) obtained from valproic acid treated and untreated cultures were prepared in MeOH and subjected to LC-ESI-MS analysis (Fig. 7).
Similar(12)
Among the phosphatases, PP1 has been identified in P. falciparum (PfPP1c) and it accounts for the major phosphatase activity in total parasite extracts [ 1, 17, 18].
Electrical conductivity and pH were determined on water extracts (1 5 compost/water) as outlined by Anderson and Ingram (1993).
Forty milliliters of absolute ethanol was added gradually to 10 ml of water extracts (1 20 w/v).
The pH and electrical conductivity were determined in compost-water extracts (1 10 w/v) (Rhoades 1982).
The chelating activity of the various concentrations of extracts (1 10 mg/ml) for ferrous ions was measured spectrophotometrically following the ferrozine method (Dinis et al. 1994).
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