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All the solvent were filtered with the help of a filter paper, collected in 6 L conical flask and subjected to thin film rotavapor under reduced pressure at 40 °C to obtain a thick brown ethanolic extract with an extractive value of 49%%.
The % extractive value and yield of extracts was found maximum in methanol and aqueous extract due to their high polarity and easy solubility of secondary metabolites in the methanol and water.
The extractive value was 3.9%%.
The extractive value obtained was 47 and 49%% for ethanol and petroleum ether.
The water soluble extractive value indicated the presence of sugar, acids, and inorganic compounds.
% of extractive value: Percent activity: The percent activity demonstrates the total anti- microbial potency of particular extract.
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The extraction time was 2 h to achieve good extractive values.
Materials and methods: Various standardization parameters like morphological characters, microscopic evaluation, physicochemical evaluations (loss on drying, ash values, extractive values), preliminary phytochemical screening and TLC chromatographic profile of the extract were carried out and the quantitative microscopy were reported.
The extractive values obtained were 27 and 22 % in ethanol and petroleum ether respectively.
The extractive values for ultrasonication with ethanol at room temp and at higher temp were 25.28 and 54.55 % and for ultrasonication with petroleum ether at room temp and at higher temp were 19.3 and 34.14 % respectively.
Extractive values of ethanol soluble, ash value, acid-insoluble ash, and moisture contents were quantified.
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