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Variation in the dataset may reflect differences in developmental stage and health between plants, but also differences in extractability of specific components (e.g. lignification might hinder extraction of wall components) and tissue- and cell-type specific differences in cell wall composition.
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No other extensins were detected in extracts of these cells, and saline extraction of walls resulted in the near complete removal of JIM11 epitope signals, indicating minor, if any network extensin prior to incubation with H2O2.
The study showed, that rumen wall extraction of arterial urea-N is subjected to both long- and short-term regulation.
Extraction of cell wall material [ 35] showed that 'Scifresh' fruitlet already had a higher proportion of more tightly bound pectin and hemicelluloses than 'Royal Gala', possibly making stronger cellulose-pectin and/or cellulose- hemicellulose networks in 'Scifresh' compared to 'Royal Gala', cell wall features that continued to exist up to the ripe stage.
Values are means from three cell wall extractions with duplicate assays each ± standard deviation.
Sequential plant cell wall extractions and glycome profiling of the different biomass samples were carried out as described previously [ 25, 30].
Remarkably, LM5 was found in high amounts in the CDTA cell wall extractions for all Equisetum-species and Danaea, suggesting that they might have distinct cell wall architectures compared to all other ferns and lycophytes studied where NaOH was required to extract the LM5 epitope.
This hypothesis is supported by the observation of ChpF and ChpH homodimers in preparations of chaplins extracted from the cell wall (Figure S3; extraction method described in Text S1).
Then thresholding is applied to the isotropic volume, based on the CT values characteristic of the colonic wall, for extraction of most parts of the colonic wall, followed by a region-growing method for extraction of the complete colonic wall.
This effect was restricted to walls which contained network GvP1, since saline extraction of H2O2-treated walls containing GvP1-free EIBMPs swelled to hydration levels similar to that observed after saline extraction of native walls.
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