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The yeast genomic DNA extraction used for the PCR reactions was performed as previously described (Drumonde-neves et al. 2013), with modifications.
Headspace extraction used for volatile analytes in solid or liquid samples is preferable technique for a quality control but the sensitivity is limited (Pawliszyn 2000).
Different extraction protocols tailor to different compound classes based on several solvents (e.g., methanol/water/chloroform mixture used for simultaneous extraction of both hydrophilic and hydrophobic compounds or solid phase extraction used for volatile metabolites [40]), limiting coverage of the metabolome (Figure 1B).
For each RNA extraction used for microarrays, a sample was collected in order to perform Q-PCR experiments.
Interestingly, ALOE did not contribute to the cellular antioxidant protection by NAE-8®, suggesting that the Aloe-based extraction used for producing NAE-8® allows increased extraction of biologically relevant compounds.
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Gene expression of samples from M-ZE and LT-SE were compared using the same RNA extractions used for the microarray hybridizations.
Ultrasound-assisted extraction was used for extraction of bioactive compounds and for production of Allium ursinum liquid extract.
The efficiency and reproducibility of two techniques, Soxhlet and supercritical fluid extraction (SFE), used for extraction of PAHs from soot were compared.
Solvent extraction was used for extracting oil from this algae powder.
MicroRNAs were isolated with the extraction procedure used for the protein-coding genes (total RNA extraction).
A. thaliana proteins were extracted using a modified version of the extraction procedure used for Fraction I in [14].
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