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The experiments demonstrated the successful effect of applying eccentric agitation with UAE to provide a combination of agitation and sonication to extract the proteins in short extraction times.
However, for longer extraction times, the highest values of extracted polyphenols were obtained by conventional techniques.
While every effort was made to extract the samples as soon as possible, extraction times varied between 3 14 days during this study.
Finally, we note that the DBLModeller model extraction times do not exceed 3 minutes: extracting the model for the largest of our schemas took only 174s.
The bovine teeth were extracted from a different lot, representing different extraction times, to standardize and control for the variability of results, as reported by Nakamichi et al. who established that bonding strength increases as teeth age [20].
The least volatile, highest affinity compounds will be extracted from the headspace-liquid matrix last, therefore adequate extraction times must be observed [Pawliszyn 1999].
The optimal extraction times for 75% methanol was further investigated.
The extraction times ranged from 15 to 120 min.
Table 3 Model extraction times using DBLModeller and Gra2MoL Schema Size (KLOC) Tool Mean (Secs).
(DOCX 17 KB) 13065_2013_601_MOESM3_ESM.docx Additional file 3: Table S3: HPLC results by different extraction times.
However longer extraction times were necessary and lower yields of phenolic compounds were obtained [14].
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