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For this reason we adopted homemade cloth extraction thimbles, sewn from 100% cotton, to increase extraction throughput.
The combination of the chromatographic and electrophoretic mechanisms allowed the extraction and clean-up in one step with low organic solvent consumption, high extraction throughput and elution automation.
Compared to the previous method using manual DNA extraction and primer/probe sets targeting the GAPDH gene, this new method increased our DNA extraction throughput by over sevenfold, and lowered the lower limit of quantitation by up to eightfold.
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Rapid, high throughput extraction systems are needed to monitor viral infections in immunosuppressed patients.
Taken together, high throughput extraction and multiplex RT-qPCR assays reported in this study provided a rapid and standardized method for routine and simultaneous diagnosis of different RNA and DNA citrus pathogens.
Automated DNA extraction allows high throughput of samples (up to 72 samples/run) in a closed system greatly reducing the risk of cross contamination, and it also requires little manipulation thus reducing overall costs and hands on time.
Although automatic systems, which combine nucleic acid extraction with high throughput PCR amplification, reduce problems associated with manual sample processing and visualization, they are highly expensive (more than $140,000) and out of reach of even best clinical laboratories in the developing world.
Genomic DNAs from fungi were extracted using a rapid and high-throughput extraction method [ 26].
The procedure can be used for different Saccharomycetes yeast species and varying growth conditions, is suitable for high-throughput extraction in a 96-well format, and the resulting extracts can easily be post-processed for use in non-SDS compatible procedures like 2D gel electrophoresis.
Radiomics is the high-throughput extraction and analysis of computationally derived features from medical images [1].
The suggested high-throughput extraction protocol may be adjusted by slight modifications to handle other tissues.
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