In order to compare between extractions, technical replicate 1 within an extraction, was chosen without loss of generality for presentation here.
The study used four SAMs to assess variability due to separate total RNA extractions, technical replicates of each extraction and varying total RNA input.
Four miRNA Bead Array Plates (SAMs) were used to assess variability in patient and cell line samples due to separate total RNA extractions, technical replicates of each extraction and varying total RNA input.
There was also minor military cooperation with Sweden, including the extraction of technical data that proved invaluable to early German tank design.
To overcome the issue of low yield during genomic DNA extraction, several technical improvements were made to optimize the extraction of high-quality DNA from PM samples.
Plasma samples were prepared as described previously (10 ), and extracted nucleic acids were tested for GemyC1c DNA by using a specific PCR that included negative, positive, and extraction controls (Technical Appendix).
Unlike named entity recognition (NER) systems on e.g. the news domain, entity recognition on medical domains comprises of extractions of technical terms in the broader medical and biological arena such as name of diseases, proteins, substances and so on, see e.g. [10, 11].
The experimental approach was to look at variability between extractions, between technical replicates (both within and between plates) and between varying inputs of RNA using RNA from five colon cancer specimens and four cell lines.
The creation of this corpus was destined to the term extraction focused on technical manuals.
The goals of this study were to understand variability due to SAM, extraction, dilution and technical replication.
