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Suitable experiments were conducted and the initial finite element model was modified based on the experimental extraction strength of interference fit joints.
The screw extraction strength required for screw failure may depend on the biomechanical test settings.
The other sample was homogenized and subjected to sequential extraction buffers of increasing protein extraction strength.
Furthermore, 842 N were assumed as screw extraction strength based on the results of Hearn et al. [ 21].
Western blots of total brain protein (RIPA) and sequential extraction buffers (high salt, high salt/Triton X-100, SDS and formic acid) of increasing protein extraction strength were performed to examine solubility state.
The value was derived from the 842 N as the screw extraction strength resulting in a screw fixation failure [ 21] applied to a 50 mm long screw (the maximum screw length modelled) with a diameter of 4.5 mm (the average of 4 and 5 mm modelled screws).
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A Plackett Burman design was used to screen multiple method factors including extraction solvent strength, extraction solvent volume, shaking speed of a reciprocating shaker, and shaking time.
Even what can be objectively measured about a cup of coffee, its extraction and strength, "cannot tell you how good the coffee is…you do that by taste," Vincent Fedele, whose MoJoToGo tools are widely used in the coffee industry, wrote in an e-mail.
Parameters, including elution volume and breakthrough volume in the SPE step, temperature in the solvent conversion step, and fiber type, ionic strength, extraction temperature, extraction time, and pH in the SPME step were optimized to improve the performance of the method through either single factor comparative experiment or the orthogonal experimental design approach.
A factorial fractional design was employed in order to evaluate the influence of the equilibrium time, ionic strength, extraction time and temperature for the extraction of N-nitrosodimethylamine, N-nitrosodiethylamine, N-nitrosopiperidine and N-nitrosopyrrolidine in sausages.
Influence variables such as volume of extracting (CHCl3) and dispersing solvents (ACN), pH and ionic strength, extraction time and centrifugation time and speed were screened in a 27 4 Plackett Burman design was investigated.
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