A high current electron gun utilizing hollow cathode and washer stacks has been designed and constructed with a single pulse power system that can provide voltages for both arc discharge and extraction sequentially.
In addition, we expect that the robust performance of our Bayesian approach can also benefit from the embedded winner's curse correction in our model and the reduction of the propagations of uncertainties (introduced from performing normalization, imputation, and feature extraction sequentially rather than in one integrative step).
The concentrated residue was extracted sequentially with petroleum ether, chloroform, ethyl acetate, and n-butanol, the residue from each extraction step being used to obtain the subsequent fraction.
The pellet was then extracted sequentially with 1 ml of TBS+ containing 1% of Triton X-100, and TBS+, 1 M sucrose, following extraction with RIPA buffer (50 mM Tris-HCl, pH 7.4, 175 mM NaCl, 5 mM EDTA, 1% NP-40 and 0.5% sodium deoxycholate) and 0.1% SDS.
These extractions were sequentially carried out in TBS+, TBS+ and 1% Triton™ X-100, and RIPA buffer for the isolation of the soluble form of α-synuclein and in 8 M urea/5% SDS to collect the insoluble fraction.
We used dental explorer, in procedure of peritomy, subluxation, luxation, extraction, and curettage, sequentially.
The geochemical fractions (acid soluble (F1), reducible (F2), oxidizable (F3), and residual (F4) fraction) of the metals were sequentially extracted using the sequential extraction technique of the Commission of the European Communities Bureau of Reference (BCR).
Briefly, 10 ug/ml [C1] cholic acid was added to the serum prior to extraction and the samples sequentially extracting using equal volumes of serum with 1% ammonium hydroxide and ethyl acetate (EtOAc) four times.
Cartilage was sequentially extracted using increasing denaturants and the extraction profiles of specific proteins determined using SDS-PAGE/Western blotting.
Peptides from the gel pieces were sequentially extracted twice in 100 μl of extraction buffer [50% (v/v) acetonitrile, 0.1% (v/v) trifluoroacetic acid (TFA) in distilled water (DW)].
The main reasons can be found in the differences in the hardware architecture, in the queuing time and also in the sequentially executed extraction of the response from the FE-output files.
