Exact(4)
As the included studies reported results on efficacy in a very diverse and often incomplete manner, we performed an additional extraction round for extracting or imputing outcome data (see on-line supplemental material, section 1.2 for how outcomes were selected (Additional file 1)).
Samples were sonicated for 5 min and supernatant was pooled with an additional peptide extraction round with 50% acetonitrile/1% formic acid for 20 min at RT.
In each extraction round two negative controls were added.
Almost all available ancestors have entered the population after extracting 10 generations (between 95-99% of the number of animals in the last extraction round).
Similar(56)
After RNA extraction, two rounds of amplification and labeling, gene-expression profiles of each sample were obtained by using human whole genome (44K) Agilent microarray chips.
After two rounds of 50∶50 phenol/chloroform extraction and one round of chloroform extraction, samples were ethanol precipitated.
The reaction was stopped by adding 100 μL of 40 mM EDTA and 2% SDS, followed by two rounds of phenol-chloroform extraction and one round of chloroform extraction.
After three rounds of extraction, the extracts were analyzed by SDS PAGE.
The same steps of PCR amplification, gel extraction, a second round of PCR, and PCR purification were also used to sequence Glyma15g08680 in two homozygous wild-type and two CD-5/CD-5 individuals (primers in Table S4).
A fluid extract was prepared from the dried material by hydroalcoholic extraction in four rounds of percolation.
The DNA was then extracted by one round of phenol-chloroform-isoamylalcohol extraction and two rounds of chloroform-isoamylalcohol extraction.
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