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The differences in P forms detected with and without Chelex raise questions about previous studies that detected only orthophosphate in acid extracts of soil as part of a sequential extraction procedure for which pH was increased without using a cation exchange resin treatment [27, 28].
This is not a simple artefact of the extraction procedure: for comparison, a neutralised 6% perchloric acid extract tells a similar story about the energetic state, that is, AMP and ADP both higher in concentration than ATP, and also completely lacking a signal from phospholombricine (Additional file 9).
A sequential extraction procedure for the speciation of heavy metals and a phytotoxicity test using rice were performed.
The extraction procedure for SPE was optimized considering volume loading, pH effect, and human plasma matrix interferences.
A modified extraction procedure for wheat triticin allowed a better resolution of the triticin bands on SDS-PAGE.
The paper describes a novel extraction procedure for lipopolysaccharides (LPS) from Salmonella enterica subsp. enterica (PCM 2266).
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A response surface methodology has then been used to maximize total polyphenol content of extracts and investigate influence of parameters involved in extraction procedures for both total polyphenols content and composition of extracts.
This technique could be used to expand the extraction procedures for soil P-NMR.
It can be assumed that the results of all seven labs which use solvent extraction procedures for analysis of fish samples are comparable.
DNA was obtained from frozen peripheral blood by standard extraction procedures for all samples.
The recoveries of all three QC levels are consistent, precise and reproducible, indicating the suitability of liquid extraction procedures for separation of YH-8 from rat plasma samples.
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