Raman spectroscopy is a fast, non-destructive and reliable analytical technique for H2O2 quantification, which avoids the drawbacks of traditional iodometric determinations (sample extraction, preparation of the reagents and a long time of analysis).
Details concerning DNA extraction, preparation of DNA pools and RRL libraries have been described previously [24].
Sample extraction, preparation, and analyses were performed as previously described (Tan et al., 2011).
Briefly, after sample extraction preparation, we analyzed serum using positive-ion atmospheric pressure chemical ionization MS/MS.
Distinct laboratory areas should be reserved for each step: RNA extraction, preparation of PCR reactions, and amplification procedure.
The final product of crude extraction preparation may, depending on the particular cyanotoxin, be a concentrated and potentially lethal mixture.
JW and YLZ carried out the plant material preparation, PV infection, RNA extraction, preparation of digital expression libraries, sequence analysis, and contributed to data interpretation and manuscript writing.
RNA extraction, preparation of fluorescently labelled probes, hybridisation of microarrays and generation of raw data was performed as described previously [ 7].
Several technical advances enabled highly reproducible extraction, preparation, and LC/MS analysis, and thus permitting reliable quantification of liver samples from 60 rats.
DNA extraction, preparation of the reaction mixture, PCR amplification, and detection were conducted in different areas under a laminar flow hood.
