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Raman spectroscopy is a fast, non-destructive and reliable analytical technique for H2O2 quantification, which avoids the drawbacks of traditional iodometric determinations (sample extraction, preparation of the reagents and a long time of analysis).
Details concerning DNA extraction, preparation of DNA pools and RRL libraries have been described previously [24].
Sample extraction, preparation, and analyses were performed as previously described (Tan et al., 2011).
Distinct laboratory areas should be reserved for each step: RNA extraction, preparation of PCR reactions, and amplification procedure.
Briefly, after sample extraction preparation, we analyzed serum using positive-ion atmospheric pressure chemical ionization MS/MS.
The final product of crude extraction preparation may, depending on the particular cyanotoxin, be a concentrated and potentially lethal mixture.
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DNA extraction, library preparation and sequencing.
RNA extraction, library preparation, RNAseq and bioinformatics analysis was performed at Active Motif (Carlsbad, California, USA).
Protein extraction and preparation of samples for Western blot analysis were performed as described37.
M.Y. collected tissues, assisted in DNA extraction and preparation of the Illumina DNA libraries, and performed SSR analysis.
Traditionally, the sample extraction and preparation for reaction characterization have been performed manually.
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