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At the end of the 24 h extraction period, extracts were centrifuged at approximately 1800g for 30 min, and heavy particulates removed by decanting off the supernatant.
Also, protocatechuic acid and quercetin were not identified in the extracts collected in the extraction period of 10 30 and 10 40 min at 35 MPa.
In contrast, for the less polar polyphenols, the extraction of the more polar polyphenols did not start together with the extraction process, since they were not identified in the extracts collected in the extraction period of 0 10 min (20 and 35 MPa).
Based on a 24-central composite design and response surface methodology, the optimal operating conditions were determined to be a pressure of 6.9 MPa, a temperature of 313 K, an ethanol flow rate of 1 mL/min and a CO2 flow rate of 6.0 mL/min, providing an extracted lipid yield of 87 wt% over an extraction period of 30 min.
Dried R. japonicus (50 kg) was powdered and extracted with 75% aqueous ethanol by reflux three times (each extraction period lasted 3 h).
After 1 h extraction period, the formic acid suspension was centrifuged at 17,000×g, and the supernatant extracts (75 µl) were transferred to new tubes and quantified (15 µl) by scintillation counting for the intracellular [3H] accumulation.
Similar(29)
In addition, SFE extracts of the extraction periods from 80 to 480 min for 20 MPa and 180 480 min for 35 MPa reacted more intensively to a sprayed DPPH solution, proving the antioxidant activity of the extracts (Yang et al. 2014).
REN is the proportion of the injected water that the same ASR well can recapture during subsequent extraction periods.
Mathematical models based on the assumption of plug flow of supercritical solvent through a fixed bed of milled material were published for both extraction periods.
Third, it is presented a linear shortcut method by intervals based on Sovovà model; it predicts considering four extraction periods: delay, rapid extraction, slow extraction and depletion; delay time and solubility are evaluated using the 'tn' model.
(3) Based on gas extraction rates at the Dafosi Coal Mine, the proportions of gas extraction periods were calculated.
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