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In this work, three solvent systems for one-phase extraction of milk lipids were compared with the standard Folch method.
Moreover, the extraction of milk fat precursors appeared to be less regulated than those of the precursors of milk protein and lactose, which could partly explain why milk yield and milk protein yield decreased more than milk fat yield.
Another group found that extraction of milk during breast-feeding involved development of time-varying sub-atmospheric pressures within the infant's oral cavity, and that vacuum pressures as low as −145 mmHg may be generated [ 4].
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In our investigation, impact factors of this variation were studied in the extraction solution of milk powder using three mass spectrometers coupled with different ion source designs, and deuterium-labeled chloramphenicol (D5-CAP) was used as the internal standard.
To present the results obtained in the development of a simplified method, with a direct extraction of breast milk and detection by reverse phase high-performance liquid chromatography of retinol and α-tocopherol.
During breastfeeding, suction primarily depends on the orofacial muscles –the masseter, temporalis, medial and lateral petrigoideos and suprahyoid muscles working together in the extraction of breast milk [ 9].
A sequential strategy of experimental designs was used to optimize the analytical extraction of polyamines from milk.
The optimized p-MAA-EDMA was then employed as the sorbent in the MP-μ-SPE for extraction of sulfonamides from milk and chicken muscle samples, followed by high performance liquid chromatographic analysis with ultraviolet detection.
Recently, the biomechanics of milk extraction during breast-feeding have been investigated in detail.
In order to avoid the effect of the time of milk extraction on the P4 concentration, samples were collected not later than 1 h after the morning milking [ 23].
Milk contains minor amounts of nucleated cell in comparison to whole blood, so, after thawing at room temperature and prior to extraction of DNA, a concentration of milk samples was carried out by centrifuge at 2200 g for 5′ as reported by other authors [ 17].
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com