An amount of approximately 1 g of the dry, ground material was placed in a 100-mL glass vial with 50 mL of extraction medium, composed of either deionised water or 10%% (w/v) aqueous glycerol.
Oxylipins were extracted from plant tissues and quantified by HPLC using a method described in Fauconnier et al.[ 62]. 1 g of powdered frozen leaf tissue was extracted in 20 ml of extraction medium (isohexane/2-propanol, 3/2 (v/v), 0.0025% (w/v) butylated hydroxytoluene).
After decontamination, spores were extracted in ten ml of extraction medium followed by dilution plating and scoring for growth.
The powder was transferred into 10 ml of extraction medium that consisted of 50 mM Tris-HCl (pH 7.8), 5 mM DTT, 100 mM MgC12, 1 mM EDTA, and 2% (w/v) soluble polyvinylpyrrolidone (PVP-40).
The higher pressure differential is caused by CO2 compression, which supplies opportunity of extraction of light and medium hydrocarbons from oil and dissolution of CO2 into the oil.
Supernatants were passed through a Strata C18-E cartridge (3 cm3, 200 mg) (Phenomenex, Torrance, CA; Ref. 8B-S001-FBJ), preconditioned with 4 mL of methanol followed by 2 mL of extraction medium.
Furthermore varimax score models in terms of extraction medium compositions are simpler to interpret.
Fruit extracts were obtained by homogenizing 15 g of pineapples in an Ultraturrax (IKA, T18 Basic; Wilmington, NC, USA) for 1 min with 20 mL of extraction medium, 2 mM NaF methanol : water solution (80 : 20).
Therefore, even for smaller amounts of methanol, when chloroform is used as part of the extraction medium, an enrichment of phospholipids in the extract results.
