Sentence examples for extraction of histones from inspiring English sources

High-Salt Extraction of Histones Histones were extracted from asynchronous or M phase cells using high-salt as previously described (Shechter et al., 2007 ) with minor modifications.

Acid extraction of histones was performed according to [20].

Extraction of histones from whole insect cells [27] or mouse fibroblasts [28] has also been used, as well as extractions of normal and cancer tissues using HClO4 for isolation and characterization of the linker histone H1 variants [29], [30].

Such dips have initially been interpreted as nucleosome depletion at the TSS of transcribing genes [29], and have subsequently been interpreted to result from differential biochemical extraction of histones during chromatin preparation for ChIP in these regions of nucleosome instability [14], [31].

Acid extraction of histones was essentially performed as described (Shechter et al, 2007).

Extraction of histones, SDS PAGE and western blotting were carried out essentially as described previously (Cano et al, 1995; Clayton et al, 2000).

Extraction of histone protein was performed as described previously [ 24].

For the extraction of histone proteins, cells were collected by centrifugation and lysed with urea buffer (8  M Urea, 0.1  m NaH2PO4, 0,01  M Tris-Base, pH 8.0) at room temperature for 30 min on a rotating wheel.

Dissected imaginal wing discs from third instar larvae were homogenized and acid-extraction of histones was performed as described (Scheuermann et al., 2010).

We tried different concentrations of sulfuric acid and found that 0.33 M H2SO4 was an optimal concentration for extraction of the histones, which yielded 2 3 times as much protein as acid extraction from nuclei.

Nuclear extracts were prepared as described previously (Kaji et al, 2006), subjected to SDS PAGE and probed with antibodies listed in Supplementary Table 4. Acid extraction of bulk histones was achieved by washing nuclei in 0.1 M HCl according to the standard protocols.