RNA extraction of the ground tissue was carried out with the RNAeasy kit (Qiagen).
Following aqueous/acid (pH 2) extraction of fried ground beef (cooked at 200, 250, or 300 degrees C), XAD-2 column adsorption and elution with acetone, and acidic and basic liquid/liquid extractions, the samples are separated into six distinct peaks with preparative reverse-phase HPLC.
For the extraction, 4 g of ground leaves powder was mixed with 2 g of diatomaceous earth (Sigma, Munich, Germany), a dispersing agent and placed inside the extraction cell maintained at different oven temperatures of 50, 100 and 150 ± 1 °C.
For each RNA extraction, 750 mg of ground hypocotyls were used.
For each extraction, a quantity of ground tooth (ca. 0.2 g) was incubated for 24h at 50°C with shaking (220 rpm/min), in 4 ml of an incubation buffer consisting of 0.5M EDTA, 0.5% SDS and 3 mg/ml proteinase K. Afterwards, the DNA was purified using the QIAquick PCR Purification Kit (Qiagen, Germany), according to the manufacturer's protocol.
Chlorophyll content is generally measured after extraction of chlorophyll from ground leaves with organic solvents (Porra et al. 1989).
A sample preparation procedure included extraction of 2 g ground tobacco with 100 ml of 5 mM sulfuric acid solution for 3 h, filtration of the extract, and dilution of the filtrate 10-fold with deionized water.
Most of the coal reservoirs in China are of low-permeability, so hydraulic fracturing is widely used to improve the permeability in the extraction of gas by ground drilling.
In the experiment, SC-CO2 extraction of 15 g ground, de-shelled Jatropha seeds at a CO2 flow rate of 25 mL/min was investigated under various pressure (250 350 bar), temperature (313 333 K) and SSR (60 100) conditions.
This process was designed for incorporation in a dry-grind ethanol plant, and thus in-house ethanol was the processing solvent for the entire processing chain, from the initial extraction of the whole ground corn to the final purification steps.
