MIS is a green technology and appears as a good alternative for the extraction of fat and oils from food products.
Extraction of fat was done following Bligh and Dyer [ 6], with slight modifications by Kinsella et al. [ 7].
The concentration was determined after extraction of fat and proteins (acetone and petroleum ether extraction) by use of a radioimmunoassay kit (Coat-a-Count, DPC, Los Angeles, CA), earlier used in horses [ 29, 30].
The determination of fat content was performed with a Foss Tecator Extraction system Soxtec Avanti 2050 (Tecator Foss, Hillerød, Sweden) by continuous extraction of the fat from the material and then evaporating the solvent and drying and weighing the residue.
An approach for automated fast extraction of the fat content in bakery products based on focused microwave-assisted Soxhlet extraction (FMASE) and gravimetric determination is proposed.
Moreover, the extraction of milk fat precursors appeared to be less regulated than those of the precursors of milk protein and lactose, which could partly explain why milk yield and milk protein yield decreased more than milk fat yield.
An improved process of Soxhlet extraction assisted by microwave, called microwave-integrated Soxhlet (MIS) is proposed for the extraction of oils and fats from different food matrixes such as oleaginous seeds, meat and bakery products.
Whilst there was good correlation between the two methods, chemical extraction of tissue for fat increased the apparent % fat determined by 75%.
They may have heated the mammal bones before crushing them, to enhance the extraction of the marrow fat.
RIDASCREEN® Allergen extraction buffer is included in Allergen ELISA test kits from R-Biopharm AG and is developed for extraction of matrices with high fat content.
