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The immobilized enzyme was used for 10 cycles with appreciable productivity and intermittent AOT/isooctane treatment was found to be an effective method for extraction of excess water from MBGs which was confirmed by Karl-Fischer titration and TGA.
After 3-4 cycles, when the ester production was observed to decrease considerably, the MBGs were treated with dehydrant solution (1 M AOT/isooctane) for 24 h for extraction of excess water (by-product of esterification reaction), given 2-3 solvent washes of neat isooctane for complete removal of AOT and used for next cycle of esterification.
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This solution was used for the ascorbic acid extraction after quantitative reduction of 2,6-dichlorophenolindophenol dyestuff by ascorbic acid and extraction of the excess dyestuff using xylene.
After solubilisation at room temperature, acidification and extraction with chloroform, the percentage of excess IHg from demethylation of MeHg was 0.9 ± 0.1% (n = 4).
Solvent extraction of residual oil using excess methylethyl ketone followed by heating at 370 °C was, therefore, the most effective reactivation procedure.
With desired PCs, an increase of the extraction efficiency in excess of 46% is achieved experimentally and the 3D FDTD calculation explains this result faithfully.
Coalbed Natural Gas extraction usually results in the production of excess, or product, water, necessitating a strategy for disposal and minimizing landscape and habitat impacts.
Because of the thickness of cortical ovarian pieces, extended periods of extraction, labelling and washing were required to facilitate reagent penetration and ensure adequate washout of excess immunological probes.
Samples for transcriptome analysis were collected at 0, 2 and 24 h of excess light treatment, and rapidly frozen in liquid nitrogen prior to RNA extraction.
A terror of excess?
Accusations of excess have long followed him.
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