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We also analyzed the histone recovery after the direct acid extraction of cells by SDS-PAGE and immmunoblotting using antibodies against histones H3 and H2B.
In addition, alterations to connective tissue that occur as a consequence of age or pathology make extraction of cells from muscle even more challenging.
Total culture extraction of cells + broth using high cell density cultures typical of P. pastoris fermentations, was an efficient sampling technique for NMR analysis and provided a gold standard of intracellular metabolite levels; however, salts in the media affected the GC-MS analysis.
At a volume of 1 µl cell suspension, an aspiration speed of 7% and 50 µm elevation of the aspiration aperture extraction of cells resulted in the complete removal of feeder cells as determined by neomycin resistance gene expression (Figure 2, panel i, lane i).
The second step describes extraction of cells or tissues from the aforementioned organisms.
Following extraction of cells from IVD tissue, six samples of NP cells (Table 1) were taken for SA-β-gal staining.
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Briefly, Metabolon performed cold methanol extraction of cell pellets and these extracts were split into three aliquots.
Extraction of cell-free EPS confirmed that UW551ΔepsB produced less than 5% of the EPS made by wild-type UW551.
The extraction of cell-linked COX by using Triton X-100 was as described previously [ 9].
For extraction of cell lines DNA, phenol/chloroform method was used.
Briefly, lipid extraction of cell samples was performed using C17-ceramide and C17-S1P as internal standards.
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