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To permit determination of the cellulose and hemicellulose contents, the sample was subjected to acid detergent extraction of bulk hemicelluloses.
This is an interesting finding as surfactant extraction from the upper surface of acrylic paint films has not yet been identified as occurring from swabbing action alone; thus far publications have focussed on identifying migrated surfactant on paint surfaces and/or extraction of bulk film surfactant through immersion in water and other solvents [7],[7]].
In nearly all published works, NGS analyses of biodiversity usually involve DNA extraction of bulk samples (mixtures of co-occurring taxonomic groups), PCR amplification of targeted genetic markers, and NGS analysis for taxonomic composition.
Nuclear extracts were prepared as described previously (Kaji et al, 2006), subjected to SDS PAGE and probed with antibodies listed in Supplementary Table 4. Acid extraction of bulk histones was achieved by washing nuclei in 0.1 M HCl according to the standard protocols.
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At the same time, a demonstration of the suitable extraction of the bulk drug substance in a manner and form that does not destabilize the active ingredient or introduce any structural bias with direct reference to the original drug product is both critical and necessary.
A total of 50 different samples of 250 mg of sediments per pot were randomly collected and mixed before sampling the 250 mg of sediments to be processed for the extraction of the bulk total community RNA.
Extraction of effective pore-fluid bulk modulus from inverted elastic impedance of four angles on the basis of two-phase medium theory for the elastic impedance equation as the representation of reservoir pore-fluid information; 5.
As shown in Figs. 4a and 6a, we apply well log data to make a synthetic seismogram in noise and noise-free situations, and then generate high-accuracy and high-resolution elastic impedance of four angles based on the fast MCMC method for the direct extraction of effective pore-fluid bulk modulus parameters, testing the feasibility and noise immunity of the method.
In particular, in the low frequency regime, the hindered diffusion-controlled extraction of anions from the bulk of the film is also evident from the larger RD as compared to RCT and the difference in their magnitudes is more pronounced for the functionalized film thus confirming that functionalization is a useful method for controlling the redox response of conducting polymer films.
Also, due to bulk extraction of the DNA, genomic heterogeneity information can be diluted or masked [ 44].
Real-time PCR of bulk DNA extractions from seed has been utilized to detect adventitious presence of transgenes in maize [ 21].
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