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One hundred percent of the OMFSs indicated extractions for both molars in patient P, while all orthodontists indicated monitoring for patient B. Table 1 Frequency of diagnostic response regarding extraction, monitoring or other alternatives for lower third molars given by orthodontists (n = 28) and oral/maxillofacial surgeons (OMFSs) (n = 28).
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The questionnaire included, in addition to the basic indications, i.e., extraction or monitoring, "another conduct" option to be described by the respondents.
There are five steps in conducting a systematic review [10] which we are presenting briefly next: (a) identification of resources, (b) selection of studies, (c) study quality assessment, (d) Data extraction and monitoring progress, and (e) data synthesis.
Data extraction and monitoring of follow-up were equal for cases and controls.
In future studies, the inclusion of dedicated extraction controls monitoring the DNA extraction efficiency should be considered to further increase the quality of the data.
The extraction was monitored in two ways, by direct partitioning as well as by forming a complex with diphenylthiocarbazone (dithizone) for each metal ion in the Triton phase and measuring the colour intensity using a spectrophotometer.
Extractions were performed in a two batches (one for each sample set) with a blank (no starting material) extraction to monitor for cross-over contamination in each batch.
Prior to RNA extraction, we monitored all Drosophila lines (approximately 2000 strains) from our fly stock collection by eye for DCV infection symptoms (dark, elongated dead larvae, black dead pupae) [13].
DNA quality for each extraction was monitored by electrophoresis on 0.8% agarose gels.
Efficiency of nucleic acid extraction was monitored through amplification of RNAseP in cultured human-cell lysates incorporated into each round of specimen processing.
The efficiency of RNA extraction was monitored by the addition of 2 fmol of a synthetic miRNA, UniSp2 (Exiqon), to the denatured plasma samples.
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